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Anti EGFR(S768I)? Mouse Monoclonal Antibody

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Anti EGFR(S768I)? Mouse Monoclonal Antibody

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EGFR (S768I) 26483
[100ul]



Catalog Number:  26483
Gene Symbol:  EGFR/HER1/ERBB1
Description:   Anti EGFR(S768I) Mouse Monoclonal Antibody
Background:   EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR).
Immunogen:   A synthetic peptide from the internal region of EGFR which includes the mutation of S768I, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:1000-1:5000 
IHC: 1:50-1:200
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes EGFR (S768I), but not wild type EGFR from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant EGFR (S768I) and wildtype proteins. Purified His-tagged EGFR (S768I) protein (lane 1) and corresponding wild type protein (amino acids 702-987, lane 2) were blotted with anti EGFR(S768I) mouse monoclonal antibody (Cat. #26483).
Immunofluorescence:  
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(S768I) antibody. HEK293T cells were transfected with pCDNA3-GFP-EGFR (WT) plasmid (left column) or pCDNA3-GFP EGFR(S768I) plasmid (right column), then fixed and stained with anti- EGFR (S768I) monoclonal antibody (Cat. #26483).



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