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RAS(A146T)[26261][100ul]CatalogNumber:26261GeneSymbol:HRas
RAS(A146T) [26261]
[100ul]
| Catalog Number: 26261 |
| Gene Symbol: HRas; KRas; NRas |
| Description: Anti RAS(A146T) Mouse Monoclonal Antibody |
| Background: The A146T mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146T mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. |
| Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146T, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: |
| Purity: Purified from ascites |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes A146T mutant, but not wild-type RAS of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Download Datasheet: |
| Western blot: |
| Western blot analysis of recombinant HRAS(A146T) and wild-type proteins. Purified His-tagged RAS(A146T) protein (lane 2) and corresponding wild-type protein (lane 1) were blotted with Anti RAS(A146T) monoclonal antibody (Cat. #26261). |
| Immunofluorescence: |
| Immunofluorescence of cells expressing HRas proteins with anti HRAS(A146T) antibody. HEK293T cells were transfected with pCDNA3-GFP HRas(WT) plasmid (left column) or pCDNA3-GFP HRAS(A146T) plasmid (right column), then fixed and stained with anti HRAS(A146T) monoclonal antibody (Cat. #26261). |
| Related Products | |||
| Catalog# | Name | Size | Price |
| Anti Active Ras Mouse Monoclonal Antibody | 30μl | ￥4,800 | |
| Ras Activation Assay Kit | 20T | ￥6,800 | |
| Anti Ras Rabbit Polyclonal Antibody | 100 μL | ￥2,600 | |
| Anti Ras Mouse Monoclonal Antibody | 100 μL | ￥2,800 | |
| Anti RAS(A146V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(K117N) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(A146P) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G13V) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G13R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61R) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61L) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(Q61H) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti HRAS(G13A) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(G12S) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti RAS(A146T) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(G13D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Anti Ras(G12D) Mouse Monoclonal Antibody | 100 μL | ￥4,800 | |
| Publications: |
| 1. Gene Therapy 21, 888-896 (October 2014) |
| 2. J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136 |
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