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Anti-Abl (Y253H) Mouse Monoclonal Antibody

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Abl(Y253H)[26154][100µL]CatalogNumber:26154GeneSymbol:ABL1

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Abl(Y253H) [26154]
[100 µL]

Catalog Number: 26154

Gene Symbol: ABL1; ABL; JTK7; bcr-abl; c-ABL; p150; v-abl

Description: Anti Abl(Y253H) Mouse Monoclonal Antibody

Background: V-abl Abelson murine leukemia viral oncogene homolog 1 also known as ABL1, is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, stress response and cell migration. Deficient in Abl can cause severe disease such as leukemia.

Immunogen: A synthetic peptide from the internal region of Abl which includes the mutation of Y253H, human origin.

Applications: ELISA, WB, IHC, IF

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ ａnd Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: recognizes Abl Y253H, but not wildtype of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Download Datasheet:

Western blot:

Western blot analysis of recombinant Abl(Y253H) and wildtype proteins. Purified His-tagged Abl(Y253H) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti-Abl(Y253H) mouse monoclonal antibody (Cat. #26154).

Immunofluorescence:

Immunofluorescence of cells expressing Abl proteins with anti-Abl(Y253H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Abl (WT) plasmid (left column) or pCDNA3-GFP-Abl(Y253H) plasmid (right column), then fixed and stained with anti-Abl(Y253H) monoclonal antibody (Cat. #26154).

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