Anti-EEA1抗體,早期內(nèi)吞體相關(guān)蛋白1抗體實驗說明

研究領(lǐng)域 細胞生物  神經(jīng)生物學  信號轉(zhuǎn)導  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human, Mouse, Rat, Dog, Cow, hamster,
產(chǎn)品應用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 162kDa
細胞定位 細胞漿 細胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human EEA1
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
訂購:或（成）客服

英文名稱 EEA1
抗體名稱 Anti-EEA1抗體,早期內(nèi)吞體相關(guān)蛋白1抗體實驗說明
別    名 Early endosome antigen 1; Early endosome antigen 1, 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2.  

濃 度 1mg/1ml 
規(guī) 格 0.1ml/0.2ml(100ug/200ug) 
公司提供的抗體達一萬多種,公司擁有Complexin I/復合素1抗體一抗,二抗,標記一抗,標記二抗等抗體種類,*價供應。
多克隆抗體制備服務：應用保證型抗體制備（ELISA,Western Blot,IHC）和修飾特異性抗體制備（磷酸化,甲基化,乙酰化）。
單克隆抗體制備服務：應用保證型抗體制備（ELISA,Western Blot,IHC）和修飾特異性抗體制備（磷酸化,甲基化,乙?；?br />抗體純化服務：Protein A/G/L純化,抗原親和純化。
zui終抗體質(zhì)檢：成品抗體或抗血清,必須通過客戶要求的所有驗證（ELISA,Western Blot,IHC等）。所有的抗原和抗體驗證數(shù)據(jù)提交客戶。
免疫組化的呈色深淺可反映抗原存在的數(shù)量，可作為定性、定位和定量的依據(jù)。
1.陽性細胞染色分布有三種類型：細胞漿、細胞核、細胞膜表面。大部分抗原見于細胞漿，可見于整個胞漿或部分胞漿
2.陽性細胞分布可分為灶型和彌漫性
3.由于細胞內(nèi)含抗原量不同，所以染色強度不一。如果細胞之間染色強度相同，常提示其反應為非特異性
4.陽性細胞染色定位于單個細胞，且與陰性細胞相互交雜分布；而非特異性染色常不限于單個細胞，而是累及一片細胞。
5.切片邊緣、刀痕或褶皺區(qū)域，壞死或擠壓的細胞區(qū)，膠原結(jié)締組織等，常表現(xiàn)為相同的陽性染色強度，不能用于判斷陽性。

產(chǎn)品介紹 Early endosomes are cytoplasmic compartments that function in receiving and sorting endocytosed proteins for vesicular transport. EEA1 (early endosome antigen 1) is a peripheral membrane protein that co-localizes with the transferrin receptor and Rab5 on early endosomes. EEA1 contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. EEA1 has sequence homology to several yeast proteins that have been implicated in membrane trafficking, including Vps27, Fab1 and Vac1. Evidence suggests a possible role for EEA1 in mediating the regulatory effects of 3'-phosphoinositides on membrane trafficking.

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