小鼠視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)ELISA檢測試劑盒子科生物現(xiàn)貨供應(yīng),要想選對合適品牌的ELISA試劑盒,請您放心購買我司ZIKER子科生物品牌試劑盒,高靈敏度、重復(fù)性,強特異性、操作性,值得您的選擇,讓您有一個滿意的實驗結(jié)果!
小鼠視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)ELISA檢測試劑盒
英文名稱:mouse Optineurin (OPTN) ELISA Kit
貨號:ZK-M5564
規(guī)格: 96T/48T
保存條件:保質(zhì)期2-8℃低溫保存下,6個月的保質(zhì)期。
運輸方式:當?shù)乜蛻艨梢杂蓪iT配送人員免費送貨上門貨快遞,外地客戶當天下單當天免費快遞送貨上門。
技術(shù)服務(wù):凡購買子科生物任何一款ELISA酶聯(lián)免疫分析檢測試劑盒,都可以享受免費代測服務(wù),外地客戶可以由技術(shù)老師的指導(dǎo)下正確放置好樣本的保存方式后快遞郵寄到我公司技術(shù)部,本地客戶可以享受免費上門取樣服務(wù)!
小鼠視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)ELISA檢測試劑盒在它的研究、生產(chǎn)及使用中常常會碰到非特異性問題,子科生物技術(shù)部根據(jù)多年研發(fā)和臨床經(jīng)驗總結(jié)如下:
1、試劑盒特異性因素
1)固相載體的選擇。ELISA中常用的固相載體有微量滴定板、小珠和小試管三種,以微量滴定板zui為常見。它具有良好的吸附性能,孔底透明度高,空白值低,各板之間、同一板各孔之間性能相近。聚苯乙烯ELISA板由于原料的不同和制作工藝的差別,各產(chǎn)品的質(zhì)量差異很大。因此,在選擇試劑盒同時要對其使用的微孔板型號進行認證,評估。
2)包被物的純度。在酶聯(lián)免疫測定尤其是間接ELISA中,試劑的特異性取決于使用抗原的純度。目前由于技術(shù)條件的限制,包被用抗原或抗體的純度不可能達到100%,所以有些非特異性顯色不可避免,只能盡可能提高純度,提高特異性。目前使用的包被抗原一般為合成多肽抗原。
3)包被抗體的效價。具有高親和力和高特異性的包被抗體,是決定試劑特異性的重要方面。
4 )封閉。是ELISA中重要的一步,目的是封閉固相載體表面尚未被所占據(jù)的空隙[2],減少后續(xù)步驟中非特異性蛋白的干擾。
小鼠視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)ELISA檢測試劑盒SequaGel Complete Buffer Component of SequaGel 4, 4.25, 4.75, 6 or 8. No need to order separay. EC-841 100 ml
SequaGel Complete Buffer EC-841 200 ml
SequaGel XR Proprietary formulation for extended reads in both manual sequence gels and automated sequencers. EC-842 450 ml
SequaGel XR EC-842 1 liter
SequaGel XR Concentrate Proprietary formulation for extended reads in both manual sequence gels and automated sequencers. EC-843 100 ml
SequaGel XR Concentrate EC-843 450 ml
SequaGel MD Monomer Solution Proprietary solution for the resolution of minor mutational differences in conformational analysis. EC-845 200 ml
SequaGel MD SSCP Kit Proprietary solution for the resolution of minor mutational differences in conformational analysis. Contains matrix concentrate and SSCP Stop Solution. EC-846 1 kit
SequaGel MD Heteroduplex Kit Proprietary solution for the resolution of minor mutational differences in conformational analysis. Contains matrix concentrate and Triple Dye Loading Buffer. EC-847 1 kit
SequaGel MD SSCP Stop Solution Formamide loading buffer. EC-848 1.2 ml
AccuGel 19:1 (30%) 30% stock molecular biology grade solution of acrylamide/bisacrylamide, 19:1 ratio. EC-849 450 ml
AccuGel 19:1 (30%) EC-849 1 liter
AccuGel 19:1 (40%) 40% stock molecular biology grade solution of acrylamide/bisacrylamide, 19:1 ratio. EC-850 450 ml
AccuGel 19:1 (40%) EC-850 1 1iter
AccuGel 29:1 (30%) 30% stock molecular biology grade solution of acrylamide/bisacrylamide, 29:1 ratio. EC-851 450 ml
AccuGel 29:1 (30%) EC-851 1 liter
AccuGel 29:1 (40%) 40% stock molecular biology grade solution of acrylamide/bisacrylamide, 29:1 ratio. EC-852 450 ml
AccuGel 29:1 (40%) EC-852 1 liter
Triple Dye Loading Buffer (6X) Three dyes (Xylene Cyanol, Bromophenol Blue, and Orange G) allow continuous monitoring of electrophoretic runs. EC-855 1.2 ml
UreaGel Loading Buffer EC-857 10 x 1 ml
TBE 10X Commonly used gel and tank buffer for DNA electrophoresis. EC-860 1 liter
TBE 10X EC-860 4 liters
TBE 5X Commonly used gel and tank buffer for DNA electrophoresis. EC-861 1 liter