人多巴色素異構(gòu)酶(DT)ELISA檢測(cè)試劑盒●反應(yīng)時(shí)間: 1-5h
●所需樣本體積: 50-100ul
●檢測(cè)波長(zhǎng): 450 nm
●用途: For research use only. Not for diagnostic use.
●特點(diǎn):靈敏性高、特異性強(qiáng)、重復(fù)性好
●服務(wù):子科生物所有的elisa試劑盒均可免費(fèi)代測(cè),全程Elisa實(shí)驗(yàn)技術(shù)指導(dǎo)。
●運(yùn)輸:全國(guó)各地快遞免費(fèi)送貨上門,低溫運(yùn)輸,
人多巴色素異構(gòu)酶(DT)ELISA檢測(cè)試劑盒子科生物研發(fā)生產(chǎn),深圳子科生物ELISA試劑盒優(yōu)質(zhì)供應(yīng)商,擁有10年的研發(fā)和生產(chǎn)經(jīng)驗(yàn),擁有*的儀器設(shè)備,專業(yè)的技術(shù)人員和*的實(shí)驗(yàn)技術(shù),目前已經(jīng)建立強(qiáng)大的實(shí)驗(yàn)技術(shù)服務(wù)平臺(tái),品牌設(shè)立為:ZIKER 。
人多巴色素異構(gòu)酶(DT)ELISA檢測(cè)試劑盒標(biāo)本的處理方法
可用作 ELISA 測(cè)定的標(biāo)本十分廣泛,體液(如血清)、分泌物(唾液)和排泄物(如尿液)、 細(xì)胞培養(yǎng)上清、細(xì)胞、組織等均可作標(biāo)本以測(cè)定其中某種成份。有些標(biāo)本可直接進(jìn)行測(cè)定,有些 則需經(jīng)預(yù)處理。
● 血清:
室溫血液自然凝固 10-20 分鐘后,離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。收集上清。如 有沉淀形成,應(yīng)再次離心。
● 血漿:
應(yīng)根據(jù)試劑盒的要求選擇 EDTA 、檸檬酸鈉或肝素作為抗凝劑,加入 10 %( v/v )抗凝劑 ( 0.1M 檸檬酸鈉或 1% heparin 或 2.0%EDTA.Na2) 混合 10-20 分鐘后,離心 20 分鐘左右 ( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。如有沉淀形 成,應(yīng)再次離心。
●尿液、胸腹水、腦脊液:
用無菌管收集。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。如有沉淀形成, 應(yīng)再次離心。
●細(xì)胞培養(yǎng)上清:
檢測(cè)分泌性的成份時(shí),用無菌管收集。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集 上清。檢測(cè)細(xì)胞內(nèi)的 成份時(shí),用 PBS ( PH7.2-7.4 )稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到 100 萬 /ml 左右。通過反復(fù)凍融,以使細(xì)胞破壞并放出細(xì)胞內(nèi)成份。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
●細(xì)胞:
檢測(cè)細(xì)胞的成份時(shí),對(duì)于貼壁細(xì)胞,去除培養(yǎng)液,用PBS、理鹽水或無血清培養(yǎng)液洗一遍。加 入適量裂解液。用槍吹打數(shù)下,使裂解液和細(xì)胞充分接觸。通常裂解液接觸細(xì)胞10 秒后,細(xì)胞 就會(huì)被裂解。對(duì)于懸浮細(xì)胞,離心收集細(xì)胞,用PBS、理鹽水或無血清培養(yǎng)液洗一遍.加入適 量裂解液, 用槍吹打把細(xì)胞吹散。用手指輕彈以充分裂解細(xì)胞。充分裂解后應(yīng)沒有明顯的細(xì)胞沉 淀。如果細(xì)胞量較多,必需分裝然后再裂解。充分裂解后,10000-14000g離心3-5 分鐘,取上 清,即可進(jìn)行后續(xù)操作。
●組織標(biāo)本:
切割標(biāo)本后,稱取重量。加入一定量的 PBS 或組織蛋白萃取試劑,緩沖液中可加入蛋白酶抑制 劑。用手工或勻漿器將標(biāo)本勻漿充分。離心 20 分鐘左右( 2000-3000 轉(zhuǎn) / 分)。仔細(xì)收集 上清置于 -20 度或 - 70 度保存,如有必要,可以將樣品濃縮干燥。分裝后一份待檢測(cè),其余冷凍備用。
注意:客戶收到我公司產(chǎn)品請(qǐng)*時(shí)間確認(rèn)試劑盒是否有破損,酶標(biāo)板是否封閉,使用前 TMB顯色液應(yīng)為無色透明溶液,若發(fā)現(xiàn)顏色異常,或者其他問題請(qǐng)及時(shí)與我司售后人員進(jìn)行,我們會(huì)*次安排進(jìn)行退換。
封板膜只限一次性使用,以避免交叉污染,底物請(qǐng)避光保存。使用之后所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理,以免造成污染!
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