人尿激酶型纖溶酶原激活物(uPA/PLAU)ELISA檢測試劑盒
?品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
?有效期: 4 個月(4℃);8 個月(-20℃)。
?保存方法: 2-8℃(頻繁使用時); -20℃(長時間不用時)。
?服務:子科生物所有的elisa試劑盒均可免費代測,全程Elisa實驗技術指導
人尿激酶型纖溶酶原激活物(uPA/PLAU)ELISA檢測試劑盒子科生物現(xiàn)貨供應,子科生物進口elisa試劑盒,國產(chǎn)elisa試劑盒,elisa試劑盒說明書,elisa試劑盒免費代測,ELISA試劑盒技術服務,如需Elisa試劑盒說明書及報價,可我司。
人尿激酶型纖溶酶原激活物(uPA/PLAU)ELISA檢測試劑盒標本的采集及保存:
1.血清:標本請于室溫放置2小時或4℃過夜后于1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃保存,但應避免反復凍融。
2.血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內(nèi)于2 - 8° C 1000 x g離心15分鐘,或將標本放于-20℃保存,但應避免反復凍融。
操作步驟
各試劑在使用前平衡至室溫。
1. 加樣:分別設空白孔、標準孔、待測樣品孔。除空白孔外,余孔分別加標準溶液或待測樣品100ul,注意不要有氣泡,輕輕混勻,酶標板加上蓋,37℃反應120分鐘。
2. 棄去液體,甩干,不用洗滌。
3. 每孔加檢測溶液A工作液 100ul,37℃,60分鐘。洗板3次,350ul/每孔,甩干。
4. 每孔加檢測溶液B工作液 100ul,37℃,60分鐘,洗板5次,甩干。
5. 依序每孔加底物溶液90ul,37℃避光顯色30分鐘(此時肉眼可見標準品的前3-4孔有明顯的梯度蘭色,后3-4孔梯度不明顯)。
6. 依序每孔加終止溶液50ul,終止反應(此時蘭色立轉黃色)。用酶聯(lián)儀在450nm波長依序測量各孔的光密度(OD值)。
洗板方法:
手工洗板方法:吸去(不可觸及板壁)或甩掉酶標板內(nèi)的液體;在實驗臺上鋪墊幾層吸水紙,酶標板朝下用力拍幾次;將推薦的洗滌緩沖液至少0.4ml注入孔內(nèi),浸泡1-2分鐘,根據(jù)需要,重復此過程數(shù)次。
自動洗板:如果有自動洗板機,應在熟練使用后再用到正式實驗過程中。
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