小鼠堿性磷酸酶(ALP)ELISA試劑盒子科生物現(xiàn)貨供應,子科生物進口elisa試劑盒,國產(chǎn)elisa試劑盒,elisa試劑盒說明書,elisa試劑盒免費代測,ELISA試劑盒技術服務,如需Elisa試劑盒說明書及報價,可我司。
小鼠堿性磷酸酶(ALP)ELISA試劑盒●品牌:子科生物ZIKER,美國R&D,美國immonoway,美國sciencell,德國IBL
●產(chǎn)品說明書:可添加在線客服索要原版詳細操作說明書,或者留下您的,我們會將產(chǎn)品詳細說明書發(fā)送至您的
●價格:電議,或咨詢在線客服,或者以郵件形式發(fā)到我司zikerbio進行詢價,我們將在*時間回復您!深圳子科生物提供大量品牌進口原裝,分裝,以及穩(wěn)定性強的國產(chǎn)ELISA試劑盒,性價比*。咨詢!
小鼠堿性磷酸酶(ALP)ELISA試劑盒標本的保存
一般說來,在 5 天內(nèi)測定的標本可放置于 4℃,超過一周測定的需低溫凍存。 對收集后當天進行檢測的標本,儲存在 4 ℃ 備用,如有特殊原因需要周期收集標本, 將標本及時分裝后放在 -20 ℃ 或 -70 ℃ 條件下保存。避免反復凍融。 標本 2 -8 ℃ 可保存 48 小時, -20 ℃ 可保存 1 個月。 -70 度可保存 6 個月。部分標 本也可加入適當防腐劑,激素類標本需添加抑肽酶。
標本的處理
可用作 ELISA 測定的標本十分廣泛,體液(如血清)、分泌物(唾液)和排泄物(如尿液)、 細胞培養(yǎng)上清、細胞、組織等均可作標本以測定其中某種成份。有些標本可直接進行測定,有些 則需經(jīng)預處理。
● 血清:
室溫血液自然凝固 10-20 分鐘后,離心 20 分鐘左右( 2000-3000 轉 / 分)。收集上清。如 有沉淀形成,應再次離心。
● 血漿:
應根據(jù)試劑盒的要求選擇 EDTA 、檸檬酸鈉或肝素作為抗凝劑,加入 10 %( v/v )抗凝劑 ( 0.1M 檸檬酸鈉或 1% heparin 或 2.0%EDTA.Na2) 混合 10-20 分鐘后,離心 20 分鐘左右 ( 2000-3000 轉 / 分)。仔細收集上清。如有沉淀形 成,應再次離心。
●尿液、胸腹水、腦脊液:
用無菌管收集。離心 20 分鐘左右( 2000-3000 轉 / 分)。仔細收集上清。如有沉淀形成, 應再次離心。
●細胞培養(yǎng)上清:
檢測分泌性的成份時,用無菌管收集。離心 20 分鐘左右( 2000-3000 轉 / 分)。仔細收集 上清。檢測細胞內(nèi)的 成份時,用 PBS ( PH7.2-7.4 )稀釋細胞懸液,細胞濃度達到 100 萬 /ml 左右。通過反復凍融,以使細胞破壞并放出細胞內(nèi)成份。離心 20 分鐘左右( 2000-3000 轉 / 分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
●細胞:
檢測細胞的成份時,對于貼壁細胞,去除培養(yǎng)液,用PBS、理鹽水或無血清培養(yǎng)液洗一遍。加 入適量裂解液。用槍吹打數(shù)下,使裂解液和細胞充分接觸。通常裂解液接觸細胞10 秒后,細胞 就會被裂解。對于懸浮細胞,離心收集細胞,用PBS、理鹽水或無血清培養(yǎng)液洗一遍.加入適 量裂解液, 用槍吹打把細胞吹散。用手指輕彈以充分裂解細胞。充分裂解后應沒有明顯的細胞沉 淀。如果細胞量較多,必需分裝然后再裂解。充分裂解后,10000-14000g離心3-5 分鐘,取上 清,即可進行后續(xù)操作。
●組織標本:
切割標本后,稱取重量。加入一定量的 PBS 或組織蛋白萃取試劑,緩沖液中可加入蛋白酶抑制 劑。用手工或勻漿器將標本勻漿充分。離心 20 分鐘左右( 2000-3000 轉 / 分)。仔細收集 上清置于 -20 度或 - 70 度保存,如有必要,可以將樣品濃縮干燥。分裝后一份待檢測,其余冷凍備用。
深圳子科生物自主研發(fā)酶聯(lián)免疫分析檢測試劑盒,品牌為ZIKER,可以檢測Human,Rat、Mouse、Porcine、Sheep、Monkey、Goat、Bovine、Canine、Rabbit、Chicken、Duck、Fish、Plant 等各種樣本,品種齊全,質量保證,免費代測、技術全程支持服務,我公司長期跟各大高??蒲袑嶒炇冶3终n題合作,經(jīng)驗豐富,質量穩(wěn)定!我們所有的產(chǎn)品都是我司直接供貨,無中間差價,直接供貨,讓您以*惠的實驗預算完成您的實驗!
其他相關產(chǎn)品如下:Phospho-p53 (S315) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-p53 (S366) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-p53 (S6) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-p53 (S9) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-p63 (S395) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-p73 (Y99) Cell-Based Colorimetric ELISA Kit Human, Mouse 96 well Immunoway
Phospho-RelB (S552) Cell-Based Colorimetric ELISA Kit Human, Mouse 96 well Immunoway
Phospho-SGK1 (S422) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-SGK1 (S78) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Smad2 (S250) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Smad2 (S467) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Smad2 (T220) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Smad2/3 (T8) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Sp1 (T453) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Sp1 (T739) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-c-Src (S75) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-c-Src (Y216) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-c-Src (Y419) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Stat1 (S727) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Stat1 (Y701) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Stat3 (S727) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Stat3 (Y705) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Tau (T205) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Tau (T212) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Tau (T231) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Flk-1/Flt-4 (Y1054/Y1063) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Flk-1 (Y1059) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Flk-1 (Y1214) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Caspase-9 (Y153) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-Caspase-9 (S196) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Phospho-Caspase-3 (S150) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Caspase-1 (S376) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Phospho-Caspase-2 (S157) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Acetyl-Histone H1 (K25) Cell-Based Colorimetric ELISA Kit Human 96 well Immunoway
Acetyl-Histone H2A (K5) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H2B (K15) Cell-Based Colorimetric ELISA Kit Human, Mouse 96 well Immunoway
Acetyl-Histone H3 (K14) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H3 (K18) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H3 (K23) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H4 (K12) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H4 (K16) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-Histone H4 (K8) Cell-Based Colorimetric ELISA Kit Human, Mouse, Rat 96 well Immunoway
Acetyl-NFκB-p65 (K310) Cell-Based Colorimetric ELISA Kit Human, Mouse 96 well Immunoway