細(xì)胞名稱(chēng)

NR8383大鼠肺泡巨噬細(xì)胞

描述

NR8383 (正常大鼠，1983年8月3日)來(lái)源于肺灌洗時(shí)的正常大鼠肺泡巨噬細(xì)胞。 細(xì)胞在gerbil肺細(xì)胞連續(xù)培養(yǎng)液存在下培養(yǎng)了大約8-9個(gè)月。 隨后，不再需要外源生長(zhǎng)因子。 通過(guò)有限稀釋法從單個(gè)細(xì)胞克隆并亞克隆NR8383細(xì)胞，并三次用軟瓊脂亞克隆。 細(xì)胞表現(xiàn)出巨噬細(xì)胞的特性，吞噬酵母多糖和銅綠，非特異性脂酶活性，F(xiàn)c受體，氧化降解；分泌IL-1, TNF beta和IL-6，可重復(fù)地響應(yīng)外源生長(zhǎng)因子。 NR8383細(xì)胞響應(yīng)*，分泌TGF beta前體。 在*刺激下，TGF beta mRNA表達(dá)也上升。 細(xì)胞對(duì)內(nèi)毒素敏感。 1-10 鈉克/毫升的LPS水平抑制增生達(dá)50%。 即使達(dá)到0.001毫克/毫升的水平，LPS抑制還是無(wú)毒且在后續(xù)過(guò)程中可逆的。 NR8383細(xì)胞株提供了高響應(yīng)的肺泡巨噬細(xì)胞的均一來(lái)源，可以用于體外研究巨響細(xì)胞相關(guān)活性。

動(dòng)物種別

大鼠

性別

***

組織來(lái)源

肺；巨噬細(xì)胞；肺泡

形態(tài)

巨噬細(xì)胞

培養(yǎng)基和添加劑

F12K培養(yǎng)基(SIGMA,貨號(hào)N3520，添加NaHCO3 2.5g/L)，80%；優(yōu)質(zhì)胎牛血清，20%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

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