細(xì)胞名稱(chēng)

C6大鼠膠質(zhì)瘤細(xì)胞

描述

膠質(zhì)細(xì)胞株C6是由Benda等用N-nitrosomethylurea誘導(dǎo)的大鼠膠質(zhì)瘤克隆，并經(jīng)過(guò)一系列的體外培養(yǎng)和動(dòng)物傳代交替后建成的。 當(dāng)細(xì)胞從低密度生長(zhǎng)到滿(mǎn)瓶時(shí)，S-100產(chǎn)量增加10倍。

動(dòng)物種別

大鼠

性別

***

組織來(lái)源

膠質(zhì)瘤，腦，膠質(zhì)細(xì)胞

形態(tài)

成纖維細(xì)胞

培養(yǎng)基和添加劑

F12K培養(yǎng)基(SIGMA,貨號(hào)N3520，添加NaHCO3 2.5g/L)，82.5%；馬血清，15%；優(yōu)質(zhì)胎牛血清，2.5%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

A。僅供研究之用。

REFERENCE

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