細(xì)胞名稱

NCI-N87人胃癌細(xì)胞（2013年新引進(jìn)）（干細(xì)胞庫保藏）

描述

人胃癌細(xì)胞

動物種別

人

性別

組織來源

胃

形態(tài)

上皮樣

培養(yǎng)基和添加劑

1. 人胃癌細(xì)胞NCI-N87*培養(yǎng)液配方（100 ml）： 　　 RPMI 1640 Medium (Invitrogen, 11875-093) 90 ml 　　 FBS (Biochrom, S0115) 10 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。 （注：該細(xì)胞貼壁較慢，建議復(fù)蘇后讓細(xì)胞貼壁48h后再進(jìn)行后續(xù)實驗操作。）

供應(yīng)限制

REFERENCE

Park JG, et al. Characteristics of cell lines established from human gastric carcinoma. Cancer Res. 50: 2773-2780, 1990. PubMed: 2158397 NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. NCI-N87 is a gastric carcinoma cell line derived in 1976 by A. Gazdar and associates at the National Cancer Institute from a liver metastasis of a well differentiated carcinoma of the stomach taken prior to cytotoxic therapy. The tumor was passaged as a xenograft in athymic nude mice for three passages before the cell line was established.