細(xì)胞名稱

KB人口腔表皮樣癌細(xì)胞

描述

zui初認(rèn)為這個(gè)細(xì)胞源自口腔表皮癌，但隨后通過同工酶分析、HeLa標(biāo)記染色體和DNA指紋分析發(fā)現(xiàn)，起源細(xì)胞已被HeLa污染。 角蛋白免疫過氧化物酶染色陽性。 有報(bào)告稱KB細(xì)胞含有人乳頭狀瘤病毒18 (HPV-18)序列。

動(dòng)物種別

人

性別

***

組織來源

HeLa污染

形態(tài)

上皮細(xì)胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；胎牛血清，10%。 或 RPMI-1640(GIBCO,貨號31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Eagle H . Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. Exp. Biol. Med. 89: 362-364, 1955. PubMed: 13254761 Boshart M , et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740 Science 133: 1559, 1961. Eagle H , Foley GE . Cytotoxicity in human cell cultures as a primary screen for the detection of anti-tumor agents. Cancer Res. 18: 1017-1025, 1958. PubMed: 13596943 Proc. Soc. Exp. Biol. Med. 94: 661, 1957. Eagle H , et al. Viral susceptibility of a human carcinoma cell (strain KB). Proc. Soc. Exp. Biol. Med. 91: 361-364, 1956. PubMed: 13322936 St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Lee RJ , Huang L . Folate-targeted, anionic liposome-entrapped polylysine-condensed DNA for tumor cell-specific gene transfer. J. Biol. Chem. 271: 8481-8487, 1996. PubMed: 8626549