細(xì)胞名稱 | RWPE-1人正常前列腺上皮細(xì)胞 |
描述 | 腫瘤抑制基因: p53 + [PubMed: 9214605] pRB + [PubMed: 9214605] 一位正常男性前列腺組織切片的周圍區(qū)域的上皮細(xì)胞用單拷貝的人乳頭瘤病毒的18(HPV-18)進(jìn)行轉(zhuǎn)化,建立了RWPE-1 (ATCC CRL-11609) 細(xì)胞株 [PubMed: 9214605]. 在三維Matrigel培養(yǎng)時(shí),在雄激素作用下,RWPE-1細(xì)胞形成腺胞并向培養(yǎng)基中分泌PSA。[PubMed: 11170142]. 當(dāng)與Matrigel或基質(zhì)細(xì)胞混合注射雄性裸鼠時(shí),RWPE-1細(xì)胞也能形成腺胞[PubMed: 11304724] 并產(chǎn)生PSA。 來源于RWPE-1的細(xì)胞再用Kirstin鼠類腫瘤病毒轉(zhuǎn)染Ki-ras基因,建立了能成瘤的RWPE-2細(xì)胞株(ATCC CRL-11610) [PubMed: 9214605] 和 RWPE2-W99 (ATCC CRL-2853) 細(xì)胞株。 另外,用N-甲醇-N-硝基脲(MNU)處理RWPE-1,建立了一系列模擬前列腺癌進(jìn)程中不同時(shí)期的成瘤細(xì)胞株。 它們是 WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850, WPE1-NB11 (ATCC CRL-2851) 和 WPE1-NB26 (ATCC CRL-2852) 細(xì)胞株。 據(jù)提供者報(bào)道,RWPE-1 細(xì)胞株(ATCC CRL-11609)經(jīng)過檢測,乙肝、丙肝、人免疫缺陷病毒都呈陰性。 |
動(dòng)物種別 | 人 |
性別 | 男 |
組織來源 | 器官: 前列腺疾?。?正常細(xì)胞類型: 上皮細(xì)胞 |
形態(tài) | 上皮細(xì)胞 |
培養(yǎng)基和添加劑 | 訂購此細(xì)胞需提供100ml培養(yǎng)基。這株細(xì)胞的基本培養(yǎng)基作為Invitrogen (GIBCO)提供的kit的部分: 角化細(xì)胞無血清培養(yǎng)基K-SFM,kit目錄號(hào)17005-042。隨kit提供這株細(xì)胞生長所需的兩種添加劑(牛垂體提取物BPE及人重組表皮生長因子EGF)。 配制*生長培養(yǎng)基,需添加以下成分: 0.05 mg/ml BPE – 隨K-SFM提供 5 ng/ml EGF -隨K-SFM提供。 注意: 不要過濾*培養(yǎng)基。 氣相: 空氣,95%;二氧化碳,5%。溫度: 37.0°C。 |
供應(yīng)限制 | A。僅供研究之用。 |
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