細胞名稱 | MRC-5人胚肺細胞 |
描述 | MRC-5細胞系來自14周齡男性胎兒的正常肺組織,該細胞老化前能傳代42到46次群體倍增。 它是正常二倍體細胞系,46,XY核型。 模式染色體數(shù)為46,概率為70%。 |
動物種別 | 人 |
性別 | 男 |
組織來源 | 肺 |
形態(tài) | 成纖維細胞 |
培養(yǎng)基和添加劑 | MEM培養(yǎng)基(GIBCO,貨號41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;優(yōu)質(zhì)胎牛血清,10%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。 |
供應(yīng)限制 | A。僅供研究之用。 |
REFERENCE | Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953 Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55. Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262 Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893 MOLECULAR THERAPY 11(4):531,2005 |
暫無信息 |