細胞名稱

MRC-5人胚肺細胞

描述

MRC-5細胞系來自14周齡男性胎兒的正常肺組織，該細胞老化前能傳代42到46次群體倍增。 它是正常二倍體細胞系，46，XY核型。 模式染色體數(shù)為46，概率為70%。

動物種別

人

性別

男

組織來源

肺

形態(tài)

成纖維細胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed: 4316953 Proc. Symp. Human Diploid Cells. Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55. Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed: 12635262 Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed: 12635218 Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893 MOLECULAR THERAPY 11(4):531,2005