細(xì)胞名稱

IMR-32人神經(jīng)母細(xì)胞瘤細(xì)胞

描述

IMR-32細(xì)胞是1967年四月 W.W. Nichols, J. Lee and S. Dwight從一位13個(gè)月大的男嬰下腹部腫瘤中分離建株。 診斷認(rèn)為是神經(jīng)母細(xì)胞瘤，并有少部分區(qū)域的器質(zhì)性分化。 包括兩種類型的細(xì)胞。 主要的是小的神經(jīng)母細(xì)胞樣的細(xì)胞。 另一種是大的透明成纖維細(xì)胞。 細(xì)胞提交到ATCC時(shí)已經(jīng)傳到第36代。 已經(jīng)證明細(xì)胞可以傳代培養(yǎng)到80代以上。

動(dòng)物種別

人

性別

男

組織來(lái)源

神經(jīng)母細(xì)胞瘤,大腦

形態(tài)

神經(jīng)母細(xì)胞,成纖維細(xì)胞

培養(yǎng)基和添加劑

MEM培養(yǎng)基(GIBCO,貨號(hào)41500034，添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；優(yōu)質(zhì)胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Tumilowicz JJ ct al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30:21 [0, 1970 PubMed: 70293585 Rostomily RC et ah Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997 PubMed: 97413638 Maestrini E et ah A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Nath Acad. Sci. USA 93: 674-678, 1996 PubMed: 96149362 Hay, R. J., CaputG J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223 -227, 1988. Fleming, D.O, Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.