GIST-T1 細(xì)胞詳細(xì)說(shuō)明

【資料簡(jiǎn)介】

齊一生物細(xì)胞庫(kù) 
Designations:   GIST-T1    GIST-T1 細(xì)胞詳細(xì)說(shuō)明
Biosafety 
Level: 
1     
Medium  & 
Serum:  
See Propagation     
Organism:  Homo sapiens (human)    
Source:  Tissue: stomach  
Disease: gastrointestinal stromal tumor 
Cell Type: epithelial-like 
Growth 
Properties: 
adherent 
Ethnicity:   Asian  
Propagation:    The base medium for this cell line is formulated RPMI-1640 Medium. To make the complete 
growth medium, add the following components to the base medium: fetal bovine serum to a 
final concentration of 10%. 
 GIST-T1 細(xì)胞詳細(xì)說(shuō)明Atmosphere: air , 95%; carbon dioxide (CO2), 5%  
Temperature: 37.0°C  
Subculturing:   Protocol:   
Remove medium and rinse monolayer with PBS.Add 3.0 ml 0.05% trypsin, 0.53 mM EDTA 
and incubate at 37C for 5 minutes. To neutralize trypsin, add 3 ml solution of 0.1% soybean 
trypsin  inhibitor .Centrifuge cell suspension at 125 xg  for 5 to 10 minutes.Resuspend cell 
pellet  in  complete  culture medium.  Add  appropriate  aliquots  of  cell  suspension  to  new 
culture vessels. 
Subc*tion Ratio: Inoculate 3 to 5 X 10(3) cells/cm2  
Medium Renewal: Twice per week 
Preservation:    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO  
Storage temperature: liquid nitrogen vapor phase 
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