Anti-ZO-1/ZO1 （zonula occludens-1）抗體說(shuō)明

【資料簡(jiǎn)介】

 Anti-ZO-1/ZO1 （zonula occludens-1）抗體說(shuō)明書(shū)

 Quantity Size : 0.1ml （dilute with pH 7.4  0.01 M PBS or antibody diluent ）

Background: This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. [SUBUNIT] Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CXADR, GJD3, HSPA4 and UBN1 （By similarity）. Interacts with occludin, claudins, GJA12 and KIRREL1. Belongs to the MAGUK family.

 Anti-ZO-1/ZO1 （zonula occludens-1）抗體說(shuō)明

Specificity : · anti-ZO-1 is a rabbit monoclonal antibody unconjugated

· specific for ZO-1 of human, mouse, rat

· epitope mapping near the amino terminus of ZO-1 of human, mouse, rat

· use for western blotting, elisa, immunoprecipitation

and immunohistochemistry

· Protein A affinity chromatography purification, purity :>95%

· Isotype: IgG

· mol wt: 191kDa

Application : · Western blotting 1:100-500

· Immunohistochemistry 1:100-500

· ELISA 1:500-1000

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 Anti-ZO-1/ZO1 （zonula occludens-1）抗體說(shuō)明