ABCG2/CD338抗體,三磷酸腺苷結(jié)合轉(zhuǎn)運(yùn)蛋白G超家族成員2抗體說明書 >>>>>點擊查看
Rabbit Anti-ABCG2/CD338 antibody
Catalog Number : BYK-0662R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
Breast Cancer Resistance Protein （BCRP） is a 70 kDa ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.
ABCG2/CD338抗體,三磷酸腺苷結(jié)合轉(zhuǎn)運(yùn)蛋白G超家族成員2抗體說明書Specificity :
– Anti- ABCG2/CD338 is a rabbit polyclonal antibody unconjugated
– specific for ABCG2/CD338 of hu, rat,mo
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 72kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500
– IF=1:100-500
– Optimal working dilutions must be determined by the end user.
ABCG2/CD338抗體,三磷酸腺苷結(jié)合轉(zhuǎn)運(yùn)蛋白G超家族成員2抗體說明書Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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