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為了獲得更理想的ELISA實(shí)驗(yàn)結(jié)果，請(qǐng)您

1、在ELISA實(shí)驗(yàn)過(guò)程中，請(qǐng)配制標(biāo)準(zhǔn)品及工作液A和B，以避免由于不準(zhǔn)確稀釋而造成的濃度誤差；

2、在ELISA實(shí)驗(yàn)過(guò)程中，酶標(biāo)板在溫育時(shí)應(yīng)加膜覆蓋以避免液體蒸發(fā)；

3、在ELISA實(shí)驗(yàn)過(guò)程中，檢測(cè)液A和檢測(cè)液B，以及底物溶液在使用前，應(yīng)置于37℃溫育30分鐘待用；

4、在ELISA實(shí)驗(yàn)過(guò)程中，洗板后應(yīng)盡快進(jìn)行下一步操作，避免酶標(biāo)板長(zhǎng)時(shí)間處于干燥狀態(tài)。

In order to obtain the optimal ELISA results

1．During ELISA assay operation, Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction and avoid foaming and mix gently until the crystals have compley dissolved;

2．During ELISA assay operation, Proper adhesion of plate sealers during incubation steps is necessary;

3．During ELISA assay operation, Substrate, Detection Reagent A and Detection Reagent B should be incubate for 30 min at 37℃ before use;

4．During ELISA assay operation, After the every step of aspiration / wash, please do not delay entering into the next step in order not to let the assay plate "too dry".