Description
Application AMV reverse transcriptase synthesizes DNA complementary （cDNA） to RNA templates. A DNA primer complementary to the RNA template and a divalent cation, either Mg or Mn, are required for initiation of transcription. This enzyme is commonly used to make cDNAs from mRNA for eventual cloning or for use as probes.
Unit Definition One unit incorporates 1 nmol of dTTP into TCA precipitable material in 10 min at 37 °C using polyadenylic acid as template and oligo（dT）12-18 as a primer.
Legal Information Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction （PCR） process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
Properties
concentration 10,000-100,000 units/mL
shipped in dry ice
storage temp. −20°C
Safety
WGK Germany 1
References
reference Ausubel, F., et al. Short Protocols in Molecular Biology 3rd ed., New York, NY, USA , （1995）, 14.42-14.43
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