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技術(shù)文章

Anti-PECAM-1抗體說(shuō)明書(shū)操作

閱讀：400發(fā)布時(shí)間：2012-11-26

Goat Anti-PECAM-1抗體說(shuō)明書(shū)操作
      Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ）
Background:   Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule （NCAM） expression is observed in a variety of human tumors including neuroblastomas, rhabdomyosarcomas, Wilms’ tumors, Ewing’s sarcomas and some primitive myeloid malignancies. The intracellular adhesion molecule-1 （ICAM-1）, also referred to as CD54, is an integral membrane protein of the immunoglobulin superfamily and recognizes the β2/α1 and β2/αM integrins. PECAM-1 （plaet/endothelial cell adhesion molecule-1）, also referred to as CD31, is a glycoprotein expressed on the cell surfaces of monocytes, neutrophils, plaets and a subpopulation of T cells. VCAM-1 （vascular cell adhesion molecule-1） was first identified as an adhesion molecule induced on human endothelial cells by inflammatory cytokines such as IL-1, tumor necrosis factor （TNF） and lipopolysaccharide （LPS）. The KALIG gene encodes a nerve cell adhesion molecule （NCAM）-like protein and is deleted in 66% of patients with Kallmann’s syndrome, anosmia with secondary hypogonadism.
pecificity :   
– Anti- PECAM-1 is a goat polyclonal antibody unconjugated                
– specific for PECAM-1 of human, mouse, rat,rabbit and dog         
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype:  IgG
– mol wt:  130 kDa Goat Anti-PECAM-1抗體說(shuō)明書(shū)操作

Application :  
– Western blotting 1:100-500                
– Immunohistochemistry 1:100-500                 
– ELISA 1:500-1000                  
– Optimal working dilutions must  be determined by the end user.
Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃
 Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.
Goat Anti-PECAM-1抗體說(shuō)明書(shū)操作


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