u2os細胞

細胞名稱： 人骨肉瘤細胞（STR鑒定正確）

細胞別稱： U2OS,U-2 OS

細胞簡稱：u2os細胞

貨號：TCH-C365

規(guī)格：1×10?cells/T25 培養(yǎng)瓶

價格：1000元 (干冰運輸需另加200元干冰費)

種屬來源：人

組織來源：骨

疾病特征：骨肉瘤

細胞形態(tài)：上皮細胞樣

生長特性：貼壁生長

培養(yǎng)體系：McCoy's 5A+10%FBS+1%P/S

配套培養(yǎng)基貨號：TCH-G365

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

u2os細胞 培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細菌、真菌、支原體檢測均為陰性

u2os細胞規(guī)格

   u2os細胞系(原名2T)是由PontenJ和SakselaE在1964年從一名15歲女孩的脛骨中等分化的肉瘤中分離建立的。與WI-38共培養(yǎng)或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的補體結(jié)合試驗（CF法）未檢測到病毒。該細胞表達胰島素樣生長因子Ⅰ、Ⅱ（IGF-Ⅰ、IGF-Ⅱ）受體和骨肉瘤衍生生長因子（ODGF）。

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Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767

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Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590"