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Anti β catenin(E53K) Mouse Monoclonal Antibody

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所在地區(qū)：湖北武漢市

聯(lián)系人：樂亮明 （經(jīng)理）

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βcatenin(E53K)[26167][100µL]CatalogNumber:26167GeneSymbol:CTNNB1

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β catenin(E53K) [26167]
[100 µL]


Catalog Number:  26167
Gene Symbol:  CTNNB1, CTNNB
Description:   Anti β catenin(E53K) Mouse Monoclonal Antibody
Background:   Beta-catenin (or β catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen:   A synthetic peptide from the internal region of β catenin which includes the mutation of E53K, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Recognizes β catenin(E53K), but not wild type β catenin protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant β catenin(E53K) and wildtype proteins. Purified His-tagged β-catenin(E53K) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti β catenin(E53K) mouse monoclonal antibody (Cat. #26167).
Immunofluorescence:  
Immunofluorescence of cells expressing β catenin proteins with anti-β catenin(E53K) antibody. HEK293T cells were transfected with pCDNA3-GFP- β catenin WT) plasmid (left column) or pCDNA3-GFP-β catenin(E53K) plasmid (right column), then fixed and stained with anti β catenin(E53K) monoclonal antibody (Cat. #26167).






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