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Anti AKT1(E49K) Mouse Monoclonal Antibody

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所在地區(qū)：湖北武漢市

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AKT1(E49K)[26137][100ul]CatalogNumber:26137GeneSymbol:AKT1

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AKT1(E49K) [26137]
[100ul]


Catalog Number:  26137
Gene Symbol:  AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Description:   Anti AKT1(E49K) Mouse Monoclonal Antibody
Background:   AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
Immunogen:   A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ ａnd Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes E49K mutant, but not wild-type AKT1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Western blot:  
Western blot analysis of recombinant AKT1(E49K) and wildtype proteins. Purified His-tagged AKT1(E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti AKT1(E49K) monoclonal antibody (Cat. #26137).
Immunofluorescence:   
Immunofluorescence of cells expressing AKT1 proteins with Anti AKT1(E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP AKT1(WT) plasmid (left column) or pCDNA3-GFP AKT1(E49K) plasmid (right column), then fixed and stained with Anti AKT1(E49K) monoclonal antibody (Cat. #26137).


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Catalog#NameSizePrice
Anti AKT1 Rabbit Polyclonal Antibody 100 μL￥2600  
Anti AKT1(E49K) Mouse Monoclonal Antibody 100 μL ￥4800   


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