Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)

Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)

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?（備注：非常規(guī)分子量：400、600。精確聚合度的小分子定制。）

結(jié)構(gòu)式：

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基本信息：

=========================================?中文名稱：Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)

別名：FITC-PEG-DBCO

英文名稱：FITC-PEG-DBCO，F(xiàn)luorescein-PEG-DBCO

CAS：-

分子量：400、600、1K、2K、3.4K、4K、5K、10K

純度：90%以上

應(yīng)用簡介：

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DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.

Copper-free Click Reaction Procedures:

Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.

物理性質(zhì)：

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狀態(tài)：淡黃色到黃色固體，或者半固體，取決于分子量。

溶解性：溶于DMSO,DMF等溶劑。

保持條件：-20℃*保存，避光，干燥。

注意事項(xiàng)：取用一定要干燥，避免頻繁的溶解和凍干。

反應(yīng)性：DBCO于含有N3基團(tuán)的反應(yīng)。

技術(shù)指標(biāo)：

=========================================?純度（PEG）：90%以上

取代率（DBCO）：90%以上

取代率（FITC）：90%以上

分散系數(shù)（PDI）：小于等于1.05

分析：COA、GPC、NMR、MALDI-TOF

Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)

Dibenzocyclooctyne-聚乙二醇-熒光素 (FITC-PEG-DBCO)