輔酶A (CoA) 測試盒ECOA-100

EnzyChrom™ Coenzyme A Assay Kit
Catalog No: ECOA-100

Shipping: On Ice
Shipment: Fedex Service
Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C

輔酶A (CoA) 測試盒ECOA-100供應(yīng)商:深圳子科生物科技有限公司

深圳子科生物科技有限公司代理美國博世BioAssay Systems，折扣低，貨期短，有大量現(xiàn)貨，詳細(xì)的產(chǎn)品報(bào)價(jià)和技術(shù)信息請咨詢在線客服！

輔酶A (CoA) 測試盒ECOA-100BioAssay Systems is going green. Printed protocols and MSDSs will no longer be included with kits. Starting January 1, 2018, protocols and MSDSs will only be available online.

Application
• For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism.

Key Features
• Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 5 - 1000 μM, fluorimetric assay 3 - 100 μM CoA.

• Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day. 

Method
• OD570nm, or FL530/585nm

Samples
• Biological

Species
• All

Procedure
• 60 min

輔酶A (CoA) 測試盒ECOA-100Size
• 100 tests

Detection Limit
• 3 μM

Shelf Life
• 6 months

More Details
• Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA’s most crucial roles is the carrying and transferring of acyl groups. BioAssay Systems method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.

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