Rabbit Anti-POKEMON
Catalog Number:Rs-0871R
Quantity size:0.2ml （dilute with pH 7.4 0.01 M PBS or diluent of antibody）
Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
Pokemon（7APOK Erythroid Myeloid Ontogenic factor）
Specificity : ·anti-pokemon is a rabbit monoclonal antibody unconjugated
·specific for pokemon of human, rat, mouse
·use for western blot, elisa, immunoprecipitation
and immunohistochemistry
·affinity chromatography purification, purity :>95%
·isotype: IgG
·mol wt: 80-85kDa
Rabbit Anti-POKEMON
Application :· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Western blotting 1:100-500
· Optimal working dilutions must be determined by the end user
Storage: Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti-POKEMON
