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【文獻(xiàn)標(biāo)題】Effects of glyphosate-based herbicide-contaminated diets on reproductive organ
toxicity and hypothalamic-pituitary-ovarian axis hormones in weaned piglets
【作者】Huiyang Fu, Feng Gao, Xiaoxu Wang, et.al
【作者單位】東北農(nóng)業(yè)大學(xué)（ Northeast Agricultural University）【文獻(xiàn)中引用產(chǎn)品】
豬總抗體氧化能力（T-AOC）ELISA試劑盒
豬超氧化物歧化酶(SOD)ELISA試劑盒
豬谷胱甘肽過氧化物酶(GPX)ELISA試劑盒
豬過氧化氫酶（CAT）ELISA試劑盒
豬谷胱甘肽S轉(zhuǎn)移酶（GST）ELISA試劑盒
豬還原型谷胱甘肽（GSH）ELISA試劑盒
豬丙二醛（MDA）ELISA試劑盒
豬過氧化氫(H2O2)ELISA試劑盒
【關(guān)鍵詞】 glyphosate-based herbicide, weaned piglets, uterus, ovary, hypothalamic-pituitary-ovarian
【DOI】doi.org/10.1016/j.envpol.2020.115596
【影響因子(IF)】6.7
【出版期刊】《Environmental Pollution》
【產(chǎn)品原文引用】
Antioxidant level of uterus
A 1 g uterine sample was taken from -20 °C and mixed with 9 ml of phosphate-buffered saline (PBS, 0.01 mol L-1 170 , PH = 7.2-7.4). After 5 min of homogenization with a 5000-rpm high-speed homogenizer, and 10% tissue homogenate was prepared. This was centrifugated at 3500 rpm for 10 min, and the supernatant was taken for the detection of the antioxidant index. Kits for measuring the total protein(Code: JA10245), total antioxidant capacity (T-AOC) (Code: J-13106), superoxide dismutase (SOD) (Code: J-50065), glutathione peroxidase (GPx) (Code: J-53063), catalase (CAT) (Code: J-52619), glutathione S-transferase (GST) (Code: J-52618) enzyme activity and reduced glutathione (GSH) (Code:J-53087), malondialdehyde (MDA) (Code: J-58063), and hydrogen peroxide (H2O2) (Code: J-35958) content in tissues were purchased from Shanghai Jinma Laboratory Equipment Co., Ltd. (Jinma, Shanghai, China). All steps were performed in strict accordance with the kits’ instructions. The measured concentrations were based on the total protein mass of the uterus.
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