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【文獻(xiàn)標(biāo)題】Zearalenone-induced aberration in the composition of the gut microbiome and function impacts the ovary reserve
【作者】Shaojing Tan , Wei Ge , Junjie Wang ,et.al
【作者單位】青島農(nóng)業(yè)大學(xué)（Qingdao Agricultural University）
【文獻(xiàn)中引用產(chǎn)品】
小鼠繆勒管抑制物質(zhì)/抗繆勒管激素(MIS/AMH)ELISA試劑盒
小鼠雌二醇(E2)ELISA試劑盒
小鼠促卵泡生長(zhǎng)激素（FSH）ELISA試劑盒
小鼠黃體素釋放激素(LHRH)ELISA試劑盒
【關(guān)鍵詞】Zearalenone，Gut microbiome，Lysophosphatidylcholines，Reproductive disorder
【DOI】doi.org/10.1016/j.chemosphere.2019.125493
【影響因子(IF)】5.77
【出版期刊】《Chemosphere》
【產(chǎn)品原文引用】
Enzyme linked immunosorbent assay
Plasma from each mice was collected to evaluate the relative content of anti-mullerian hormone (AMH), 17 b-estradiol (E2),follicle-stimulating hormone (FSH), and luteinizing hormone (LH) using ELISA kit (Jinma, Shanghai, China) following the manufacturer’s instruction. Briefly, the standardy and serum samples were added and adsorbed to wells of an ELISA plate, the enzyme-labeled plate was covered with microplate sealers and incubated at 37 C for 30 min. Then the wash buffer was used to wash plate for five times (30 s per time), 50 ml horseradish peroxides (HRP)-conjugated reagent was added into each well and incubated at 37 C for 30 min, After that the plate was washed 5 times again, and each well was added 50 ml TMB substrate A&B solution in turn and incubated at 37 C for 10 min. Finally, equal volumes of stop solution were added into each well, the absorbance was tested andcalculated in 15 min by Multiscan Spectrum (450 nm).
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