血小板衍生生長因子（PDGF）檢測試劑盒（血清.血漿.組織）Human plaet-derived growth factor,PDGF ELISA Kit 齊一生物科技（上海）有限公司是一家專業(yè)銷售酶聯(lián)免疫試劑盒,抗體蛋白、生化試劑,標(biāo)準(zhǔn)品和對照品的大型化學(xué)科技公司.自公司成立以來,本著始終擁有的創(chuàng)業(yè)激情,公司的銷售額保持高速增長,企業(yè)規(guī)模不斷擴(kuò)大

血小板衍生生長因子（PDGF）檢測試劑盒（血清.血漿.組織）操作步驟
實(shí)驗(yàn)開始前，請?zhí)崆芭渲煤盟性噭噭┗驑悠废♂寱r，均需混勻，混勻時盡量避免起泡。每次檢測都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測樣品孔?？瞻卓准訕悠废♂屢?00μl，余孔分別加標(biāo)準(zhǔn)品或待測樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加*標(biāo)記抗體工作液 100μl（取1μl*標(biāo)記抗體加99μl*標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標(biāo)記親和素工作液（同*標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長依序測量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測。
9015919    Valve, magnet, dispenser, BR8000 V2    
9015884    Incubator Tower, RCS1    
9015810    Precision Pipetting Upgrade Kit    
9015555    Cable , Clamp Motor, highflex, BR8000RD    
9019161    Gasket - Tips, PyroQ96MD    
9019152    PCB - Instrument Control, PyroQ96MD    
9014137    Spare parts tubing kit Digene    
9014094    Vacuum manifold, strip kit    
9021161    Sample Tray, QE    
9021108    Skin Panel, Top, DCU    
9021011    Controller, Y Axis, DCU    
9020997    ZS Pipetting Module, DCU    
9020909    Cable, UV curly cord, SMC, QGX    
9020908    Hinge, lid, SMC, QGX    
9020643    Waste chute, straight, AS, Cabinet Qsym    
9020632    Kit, hinges (4), Cabinet Qsym    
9020629    Door, waste, SP, Cabinet Qsym    
9020625    Kit, repair, Cabinet Qsym    
9020332    EPROM - Motherboard, RGQ    
9020233    Foot assembly, rear, CAS    
9020158    Insulation disc - chamber bottom, RG3    
9020096    Locking Pin, TLyser 2    
9020085    Thrust Washer, TLyser    
9239498    V-Housing, LX100    
9239490    EX Laser Driver, Uniphase    
9239390    PCR Plate Adapter, M96    
9236510    BR 3000, PM Service    The Overhaul covers all travel and labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked and inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.
9232764    Disposable Troughs, 20 / 30 ml (10)    Troughs holding up to 20 ml of liquid; pack of 10
9236472    BR 9600, Full Agreement    The Full Agreement 9600 provides full service coverage for the BioRobot 9600. Costs for service visits are fully covered including travel, labor, and parts. The agreement also includes one annual preventive maintenance visit, with costs for labor and parts covered. With a Full Agreement, there is a priority response time of 48 hours (2 working days).
9236443    BR, Installation    The Installation, BR Universal facilitates the quick implementation of the BioRobot into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of the BioRobot Universal instrument and provides the user with an introductory training on operation and user maintenance. The installation includes labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
9239155    Assembly, vertical axis, TWI    
9239108    Assy, Sample Needle, Actuator linkage    
9232693    MTP-Plate Holder  2.01    
9232629    Bottle, 500-ml, yellow fitting    
9232602    Vacuum manifold, plate holder bottomless    
9232579    Cooling block, 1,5ml    
9238951    Locking lever, BS15    
9238798    Rectangular bucket, sealable with cap    
9238794    Angle rotor 6 x 250 ml    
9016913    Tubing, press relief valve inlet DISU v2    
9238942    Keyboard-Display assembly, BS15    
9238753    Screwdriver, hex2mm, z-drive BR8    
9238916    Power supply 40W, BS96    
9238748    Coupling, CPC, screw, male, V2    
9238716    Seal for lid, 1350 mm    
9238898    Front panel, BS15    
9238699    Frontpanel w. keyboard foil (Zent1)    
9231793    Motor, gripper, handler, BR8000    
9238690    Computer board Zent2, no Eprom    
9231434    Probe, 0,4mm, ceramic coated for PK80    
9020071    Keypad, TLyser    
9020070    Controller 100-120V/200-240V, TLyser    
9014996    Shaker, Highspeed Unit, BR8 RD    
9014870    X-Motor, BR8    
9014790    Tip Disposal Bags MDx    Autoclavable bags for collection and disposal of used disposable tips, pack of 15
9014642    System Reagent Troughs (Qty 10)    
9019188    Mirror, PyroQ96    
1064181    EGFR RGQ PCR Kit (24) IUO    
1017647    Carrier RNA(poly A) 12x1350μg-15-25°C,KG    
1059454    ResPlex II Plus Panel (25) PRE    
Instrument Part    9019767    Sensor, interlock1, with cable, QX
Instrument Part    9019536    Pipetting Station RD, RCS
Consumable    130801    NeXtal CubicPhase 1w Mo μplate (2)
Instrument Part    9021653    Kit, stickers, eluate drawer, Qsym
Consumable    337040    Custom qBiomarker Array PreAMP Mix
Consumable    36113    Ni-NTA Magnetic Agarose Beads (6 x 1 ml)    6 x 1 ml nickel-charged magnetic agarose beads (5% suspension)
Consumable    51183    QIAamp DNA Blood Midi Kit (20)    For 20 DNA midipreps: 20 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
Instrument Part    9012916    Magnet, 96-well, Type B
Instrument Part    9233674    Probe, ceramic coat., 0.4mm, 158mm
Instrument Part    9011203    Vacuum control unit, BR8000
Instrument Part    9011860    Cooling/heating unit, BR8000 v1.01
Instrument Part    9012389    Mounting plate, level sensor, BR8000
Instrument Part    9013004    Gripper set, T-handler
Instrument Part    9013170    Tubing, syringe inlet 2, BR8000
Instrument Part    9013178    Gripper set, BR8000 v 1.00
Consumable    130706    NeXtal Tubes MPD Suite
Consumable    130711    NeXtal Tubes MBClass Suite
Accessory    990352    Filter-Tips, 1000 μl (1024)
Accessory    990452    Filter-Tips, 1000 μl wide bore (1024)
Instrument Part    9018082    LiquiChip MDD Computer
Instrument Part    9019904    PCB - Distribution, PyroQ24
Instrument Part    9019015    Cartridge rack, EZ1xl
Instrument Part    9019726    Transport lock, V2, QX
Instrument Part    9019733    Motor, Z, V2, QX
Consumable    979016    Fungi ITS2 Primer 2 For Sequencing
Instrument Part    990532    50μl Filtered Sterile Tips (960)
Consumable    51192    QIAamp DNA Blood Maxi Kit (10)    For 10 DNA maxipreps: 10 QIAamp Maxi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (50 ml)
Consumable    52304    QIAamp RNA Blood Mini Kit (50)    For 50 RNA preps: 50 QIAamp Mini Spin Columns, 50 QIAshredder Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable    75144    RNeasy Midi Kit (50)    50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers
Instrument Part    9238479    Motor, syringe, RapidPlate
Consumable    132538    NeXtal Stock Dioxane (50)    * v/v Dioxane - 50mL (5 x 10 ml tube)
Consumable    132625    NeXtal Stock TRIS. HCl (50)    1M Tris(hydroxymethyl)aminomethane HCl - 50mL (4 x 12,5 ml tube)
Instrument Part    9019573    Belt, X-axis assembly, CQx
Instrument Part    9019598    Lead, vac trap sensor, CX1
Instrument Part    9019603    Pins, locating, Mix and Reagent set, CG1
Instrument Part    9019626    Tip ejector, Cx2
Consumable    9019073    PyroMark Plate Bin, PyroQ96MD
Instrument Part    9019653    Base Plate VPT, PyroQ96VW
Consumable    970022    PyroMark Q24 CpG MLH1 (4 x 24)    For quantification of methylation level in region -209 to -188 from the transcription start site.
Consumable    990391    Rack Labeling Strips
Consumable    218412    Mouse miScript Primer Assay Set VXX.X
Consumable    1027416    FlexiTube GeneSolution siRNA 1 nmol
Instrument Part    9019011    Motor P, EZ1xl
Instrument Part    9019013    Motor Z, EZ1xl
Instrument Part    9019640    Temperature Controller, EZ1xl
Instrument Part    9019730    Board, smart key, V2, QX
Instrument Part    9019760    Power supply, high voltage, V2, QX
Consumable    978705    PyroMark PCR Kit (800)
    950240    Binding Additive (VX) (4.0 g)
    950254    Wash Reagent (VXW) (650 ml)
    990542    200μl Filtered Sterile Tips (960)