Anti- Caspase-12抗體說明書
From:ABCAM
Catalog Number :RS- 1105R
Quantity size : 0.1ml (dilute with pH 7.4 0.01 M PBS or antibody diluent ) Background: Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011].
.Specificity : Anti- Caspase-12 is a rabbit monoclonal antibody
specific for Caspase-12 of Human, Mouse, Rat,
use for western blotting,elisa,immunoprecipitation and immunohistochemistry
- Protein G affinity chromatography purification, purity :>95%
- Isotype: IgG
- mol wt: 210kDa
- Application :
- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- KLH conjugated synthetic peptide derived from the middle of human Caspase-12
- not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user..
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
