細(xì)胞名稱(chēng)

C3H/10T1/2小鼠纖維原細(xì)胞（干細(xì)胞庫(kù)保藏）

描述

小鼠纖維原細(xì)胞

動(dòng)物種別

小鼠，品系C3H

組織來(lái)源

胚胎

形態(tài)

成纖維細(xì)胞樣

培養(yǎng)基和添加劑

Eagle MEM (Invitrogen, 11090081) 87 ml FBS (Biochrom, S4115) 10 ml Glutamax（invitrogen 35050） 1 ml SODIUM PYRUVATE SOL（invitrogen 11360070） 1 ml Non-essential Amino Acids, 100? (Invitrogen, 11140) 1 ml 氣相：空氣，95%；二氧化碳，5%。溫度：37攝氏度。

供應(yīng)限制

REFERENCE

Reznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800 Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940 Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242 Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183 Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619 Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355 Jain MK, et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582