上海研盟生物科技有限公司Anti-TRAF6抗體,腫瘤壞死因子受體相關(guān)因子6抗體*，主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說明書隨貨發(fā)送，您也可以直接我司在線客服索取?？头?/p>

簡單介紹：

中文名稱：腫瘤壞死因子受體相關(guān)因子6抗體

英文名稱：Anti-TRAF6

產(chǎn)品編號(hào)：byk-1184R

產(chǎn)品別名：TNF receptor-associated factor 6; Interleukin 1 signal transducer; MGC 3310; MGC3310; RING finger protein 85; RNF 85; RNF85; TRAF 6; TRAF-6; TRAF6_HUMAN.     

產(chǎn)品規(guī)格：0.1ml、0.2ml、1ml

產(chǎn)品用途：科研實(shí)驗(yàn)

產(chǎn)品價(jià)格：請(qǐng)報(bào)價(jià)

說明書：請(qǐng)?zhí)砑涌头苯铀魅?/p>

抗體來源：該指標(biāo)有兩種產(chǎn)品，一種是兔來源抗體，一種是鼠來源抗體

克隆類型：兔來源為多克隆抗體，鼠來源單克隆抗體

交叉反應(yīng)：請(qǐng)索取說明書查看

性    狀：Lyophilized or Liquid

濃    度：1mg/1ml

亞    型:IgG

純化方法: affinity purified by Protein A

儲(chǔ) 存 液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

保存條件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-TRAF6抗體,腫瘤壞死因子受體相關(guān)因子6抗體產(chǎn)品介紹：The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.

抗原修復(fù)，必須注意以下問題：

1、修復(fù)液的選擇。
0.01M枸櫞酸（pH6.0）：應(yīng)用zui多的抗原修復(fù)液，適用于大多數(shù)的抗原，用該液修復(fù)后的抗原表達(dá)增強(qiáng)，抗原的定性和定位很理想，結(jié)果不錯(cuò);
0.05M EDTA（pH8.0）：應(yīng)用較多的抗原修復(fù)液，修復(fù)能力較強(qiáng)，對(duì)于保存時(shí)間較長的切片或目的蛋白表達(dá)較弱的組織有很好的修復(fù)效果，需要控制好修復(fù)時(shí)間，否則容易出現(xiàn)較重的背景;
0.01M TBS（pH7.4）：中性修復(fù)液，適用于大多數(shù)抗原，對(duì)于核定位蛋白有較好的修復(fù)效果;
*：應(yīng)用較多的抗原修復(fù)液，適用于大多數(shù)的抗原，使用溫度37℃，對(duì)于容易脫片的切片有很好的保護(hù)作用。
*：應(yīng)用較多的抗原修復(fù)液，適用于大多數(shù)的抗原

2.抗原熱修復(fù)時(shí)應(yīng)選擇的溫度。
據(jù)實(shí)驗(yàn)認(rèn)為溫度在92℃－98℃是合適的，尤其在95℃為，這是因?yàn)椋海?）這種溫度未達(dá)沸騰，切片不容易脫離載玻片；（2）能夠解離和破壞與蛋白交聯(lián)的甲醛醛鍵等，處理時(shí)可以隨意選擇和確定抗原修復(fù)的作用時(shí)間。如果選擇高壓修復(fù)，因?yàn)闇囟容^高，時(shí)間不宜過長

3.抗原修復(fù)時(shí)有效溫度所需持續(xù)時(shí)間根據(jù)各單位的設(shè)備條件以及使用的儀器不同，抗原修復(fù)時(shí)在有效的溫度范圍內(nèi)所持續(xù)的時(shí)間也不一樣。
4.抗原修復(fù)液必須遵循自然降溫規(guī)律，否則效果不好或達(dá)不到抗原修復(fù)的目的。
5.盡量使用足量的抗原修復(fù)液，防止切片干涸。
6.切片必須附貼牢固，否則發(fā)生掉片。

相關(guān)產(chǎn)品：

Rbms3 protein(RNA binding motif, single stranded interacting protein)  Rbms3 protein抗體
Resistin  抵抗素抗體
RELMa(Resistin like molecule alpha)  抵抗素樣分子α抗體
Reprimo/Rprm(reprimo, TP53 dependent G2 arrest mediator candidate)  細(xì)胞質(zhì)內(nèi)的糖基化蛋白抗體
C-REL(C Rel proto oncogene protein)  淋巴細(xì)胞衍生C-型凝集素抗體
RGS2((regulator of G-protein signaling 2)  G蛋白信號(hào)轉(zhuǎn)導(dǎo)調(diào)節(jié)因子2抗體
RhoA (RhoA protein)  RhoA抗體
RhoC  RhoC抗體
RNF126（Ring finger protein 126）  環(huán)指蛋白126抗體
RNF148(Ring finger protein 148)  環(huán)指蛋白148抗體
ROCK1 (Rho-associated coiled-coil containing protein kinase 1)  Rho相關(guān)蛋白激酶1抗體
ROCK2 (Rho-associated coiled-coil containing protein kinase 2)  Rho相關(guān)蛋白激酶2抗體
RPLP0（Ribosomal protein,large,P0）  酸性核糖體磷蛋白大亞基P0抗體
ROS/c ros(ros protein)  活性氧簇ROS抗體
pRb/p105-Rb（phospho ser780）  磷酸化成視網(wǎng)膜細(xì)胞瘤抑癌蛋白抗體
RRAD (Ras-related associated with diabetes )  RRAD抗體
BYKV（respiratory syncytial virus）mouse monoclonal  呼吸道合胞病毒單克隆抗體
RXRα（retinoid-X receptor alpha）  核受體RXRα抗體
Runx3(CBFA3)  新型抑癌基因/一種新發(fā)現(xiàn)的抑癌基因抗體
S6PDH(NADP sorbitol-6-phosphate deYMdrogenase)  抗6-磷酸*脫氫酶抗體
S100B  S100B蛋白抗體
S100B(S100 calcium binding protein B)  S100B蛋白抗體